Antioxidant effect of standardized extract of propolis.


Evid Based Complement Alternat Med. 2020 ;2020:7538232. Epub 2020 Oct 16. PMID: 33123209

Abstract Title: 

Antioxidant Effect of Standardized Extract of Propolis (EPP-AF®) in Healthy Volunteers: A”Before and After”Clinical Study.


Background: Propolis is rich in polyphenols, especially flavonoids and phenolic acids, and has significant antioxidant activity, shown mainly in””studies.Objective: The aim of this study was to evaluate the antioxidant efficacy and safety of a standardized propolis extract in healthy volunteers.Design: A two-phase sequential, open-label, nonrandomized, before and after clinical trial.Methods: Healthy participants received two EPP-AF® doses (375 and 750 mg/d, P.O, tid) during 7 ± 2 days, starting with the lower doses. Immediately before starting EPP-AF® administration and at the end of each 7-day dosing schedule, blood and urine samples were collected for quantification of 8-OHDG (8-hydroxydeoxyguanosine) and 8-ISO (8-isoprostanes) in urine and GSH (reduced glutathione), GSSG (oxidized glutathione), SOD (superoxide dismutase), FRAP (Ferric Reducing Antioxidant Power), vitamin E, and MDA (malondialdehyde) in plasma.Results: In our study, we had 34 healthy participants (67.7% women, 30 ± 8 years old, 97% white). The 8-ISO, a biomarker of lipid peroxidation, decreased with both doses of EPP-AF® compared to baseline (8-ISO, 1.1 (0.9-1.3) versus 0.85 (0.75-0.95) and 0.89 (0.74-1.0), ng/mg creatinine,

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