Avicenna J Phytomed. 2020 Sep-Oct;10(5):440-447. PMID: 32995322
Aqueous extract offruit pulp exhibits antihyperglycaemic activity.
Objective: Linn. () is a well-known plant used in traditional medicine. The plant is popular for its antidiabetic activity. However, effect so f its aqueous fruit pulp extract on carbohydrate hydrolyzing enzymes and its glucose uptake potential were not explored.Materials and Methods: The antidiabetic activity was assessed byα-amylase and α-glucosidase inhibitory assays after preliminary phytochemical analysis. MTT assay was carried out to find cytotoxicity. Glucose uptake activity of the extract was carried out using L6 myotubes.Results: The results showed a strongα-amylase inhibitory activity for the fruit pulp extract ofcompared to standard acarbose; the ICof the fruit pulp extract ofand acarbose was 34.19µg/ml 34.83µM. The extract also showed moderate α-glucosidase inhibitory activity. ICof the fruit pulp extract ofand acarbose were 56.91µg/ml and 45.69µM respectively. The cytotoxicity assay showed ICof>300µg/ml and ≥1000µM for the fruit pulp extract ofand metformin. The extract showed 63.99±0.08% glucose uptake in L6 myotubes whereas metformin and insulin at 10µg/ml and 10µM exhibited an uptake of 76.99±0.3% and 84.48±0.45% glucose, respectively.Conclusion: The study revealed that the fruit pulp extract ofLinn does not show any cytotoxic effect and has very goodα-amylase and good α-glucosidase inhibitory activities. The glucose uptake potential proves its postprandial hypoglycemic effect. Hence, it may be considered an antidiabetic agent for control of postprandial hyperglycemia.