Antibiotics (Basel). 2021 Jun 11 ;10(6). Epub 2021 Jun 11. PMID: 34208266
Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis.
Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of() to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of(pomegranate) and evaluated its therapeutic efficacy in rats infected with. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killingcysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number ofcysts and trophozoites in feces and intestine respectively. Interestingly, the number oftrophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controllinginfections, which suggests that it could be a viable treatment option for giardiasis.